Journal: EMBO Reports
Article Title: Phase‐separated foci of EML4‐ALK facilitate signalling and depend upon an active kinase conformation
doi: 10.15252/embr.202153693
Figure Lengend Snippet: Antibodies used for immunofluorescence (IF) and Western blotting (WB) and dilutions.
Article Snippet: Primary antibodies were against, α‐tubulin mouse (1:1,000; Sigma), α‐tubulin rabbit (1:800; 18251; Abcam), GFP (1:1,000; 6556; Abcam), GFP (1:1,000; sc‐9996; Santa Cruz Biotechnology), ALK rabbit (D5F3) (1:100; CST), ALK (31F12) mouse (1:100; CST), GRB2 rabbit (1:1,000; PA1‐10033; Invitrogen), GRB2 (1:100) mouse (C7; Santa Cruz Biotechnology), SOS1 mouse (1:100; MCA2887; Bio‐Rad;), PLCγ2 (Y759) rabbit (1:100; AP0785; ABclonal), PI3K p85β mouse (1:100; 1B180967; Abcam), c‐KIT (Y721) (1:100; 44‐494G; Thermo Fisher Scientific).
Techniques: Immunofluorescence, Western Blot